Peptide-based star polymers as potential siRNA carriers

SJ Lam; A Sulistio; K Ladewig; EHH Wong; A Blencowe; GG Qiao

Journal article

Peptide-based star polymers as potential siRNA carriers

SJ Lam, A Sulistio, K Ladewig, EHH Wong, A Blencowe, GG Qiao

Australian Journal of Chemistry | CSIRO PUBLISHING | Published : 2014

DOI: 10.1071/CH13525

Abstract

16- and 32-arm star polymers were synthesised using poly(amido amine) (PAMAM) dendrimers as multifunctional initiators for the ring-opening polymerisation (ROP) of ε-Z-l-lysine N-carboxyanhydride (Lys NCA) via the core-first approach. The resulting star polymers were subsequently post-functionalised with poly(ethylene glycol) (PEG) via carbodiimide coupling, potentially improving the biodistribution of the stars in vivo. De-protection of the carboxybenzyl (Cbz)-protected star arms yielded water-soluble cationic poly(l-lysine) (PLL) star polymers with hydrodynamic radii ranging from 2.0 to 3.3nm. Successful complexation of the PLL star polymers with double-stranded oligodeoxynucleotides (ODNs..

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University of Melbourne Researchers

Greg Qiao Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

S.J.L. acknowledges the Australian Government for providing an International Postgraduate Research Scholarship (IPRS) and an Australian Postgraduate Award (APAInt). The authors also acknowledge the Tissue Engineering Group and the Nanostructured Interfaces and Materials Science (NIMS) Group at the Department of Chemical and Biomolecular Engineering (The University of Melbourne) for instrument access. K.L. and G.G.Q. acknowledge the Australian Research Council under the Super Science (FS110200025) and Future Fellowship (FT110100411) schemes, respectively, for financial support of this work.